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流动注射-石英晶体微天平中血清蛋白对扁桃酸的手性识别

洪涛,王秋云,范军,陈文静,郑盛润,章伟光*   

  1. (华南师范大学化学与环境学院 广州 510006)
  • 收稿日期:2013-01-07 修回日期:2013-02-20 出版日期:2013-09-10 发布日期:2013-09-10
  • 通讯作者: 章伟光，教授; Tel:020-39310210; Fax:020-39310187; E-mail:wgzhang@scnu.edu.cn; 研究方向：手性分离材料的合成和色谱分析
  • 基金资助:
    国家自然科学基金(21171059)和广东省科技计划项目(2010B050300003,2010B050300021,2011B010400023)资助

Chiral Recognition of Mandelic Acid with Bovine and Goat Serum Albumins by Flow Injection Analysis-Quartz Crystal Microbalance

HONG Tao, WANG Qiuyun, FAN Jun, CHEN Wenjing, ZHENG Shengrun, ZHANG Weiguang*   

  1. (School of Chemistry and Environment,South China Normal University,Guangzhou 510006,China)
  • Received:2013-01-07 Revised:2013-02-20 Published:2013-09-10 Online:2013-09-10

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被引次数

1

摘要： 采用流动注射-石英晶体微天平(FIA-QCM)和紫外光谱法研究了牛/羊血清蛋白(BSA和GSA)与扁桃酸的手性识别。 在QCM表面，BSA和GSA的浓度分别为4.9×10-12和8.8×10-12 mol/cm2。 血清蛋白与R-扁桃酸(R-MA)作用引起QCM振动频率变化值比S-扁桃酸(S-MA)大；相应紫外结合常数也比与S-MA大，说明血清蛋白对R-MA的作用强于与S-对映体。 从识别因子分析，GSA对扁桃酸的手性识别能力优于BSA。

关键词: 血清蛋白, 扁桃酸, 流动注射-石英晶体微天平, 紫外光谱, 识别因子

Abstract: Interactions of mandelic acid(MA) and bovine/goat serum albumins(BSA/GSA) have been investigated by FIA-QCM and UV-Vis spectroscopy, respectively. Based on frequency shift of quartz crystal, surface concentrations of immobilized BSA and GSA were 4.9×10-12 and 8.8×10-12 mol/cm2, respectively. After injecting mandelic acid into FIA-QCM system, frequency of the quartz crystal decreases due to interactions between enantiomers and SA molecules and the shifts between SA and R-MA are greater than those with S-enantiomer, indicating that the interactions of SA molecules with R-MA are stronger than those with S-MA. The UV binding constants for SA with S-MA are smaller than those with R-enantiomer. Taking into account the discrimination factors(α-QCM and α-UV), GSA shows a better chiral recognition with mandelic acid than BSA.

Key words: serum albumin, mandelic acid, flow injection analysis-quartz crystal microbalance, UV-Vis spectroscopy, discrimination factor

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