中华医学超声杂志

应用化学 ›› 2011, Vol. 28 ›› Issue (06): 704-708.DOI: 10.3724/SP.J.1095.2011.00431

• 研究论文 • 上一篇    下一篇

琥乙红霉素在碳糊电极上的电化学测定方法

任艳艳,金根娣,胡效亚*   

  1. （扬州大学化学化工学院 扬州 225002）
  • 收稿日期:2010-07-29 修回日期:2010-10-08 出版日期:2011-06-10 发布日期:2011-06-10
  • 通讯作者: 胡效亚，教授; Tel/Fax:0514-87972034; E-mail:xyhu@yzu.edu.cn; 研究方向：电分析化学
  • 基金资助:
    国家自然科学基金（20875081，21075107）资助项目

Electrochemical Determination of Erythromycin Ethylsuccinate Using a Carbon Paste Electrode

REN Yanyan,JIN Gendi,HU Xiaoya*   

  1. (College of Chemistry and Chemical Engineering,Yangzhou University,Yangzhou 225002)
  • Received:2010-07-29 Revised:2010-10-08 Published:2011-06-10 Online:2011-06-10
  • Contact: JIN Gen-Di

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被引次数

5 | 1

摘要：

以碳糊电极为工作电极，采用循环伏安（CV）法和示差脉冲伏安（DPV）法研究了琥乙红霉素（EEs）在电极上的电化学行为，建立了一种测定EEs的电化学新方法。 研究结果表明，在0.1 mol/L磷酸盐（pH=8.0）的缓冲液中，EEs在0.83和0.97 V(vs.SCE）处出现2个氧化峰。 用计时安培法(I-t)对EEs进行定量分析，峰电流Ip与琥乙红霉素的浓度分别在2.0×10-7~2.8×10-6 mol/L和2.8×10-6~3.1×10-5 mol/L范围内呈良好的线性关系检出限（S/N=3）为1.0×10-7 mol/L。 采用标准加入法测得回收率为950%～988%，RSD为3.4%(n=3)。 该方法具有较高的选择性和灵敏度，可用于药剂中EEs含量的测定，结果令人满意。

关键词: 琥乙红霉素, 碳糊电极, 电化学测定, 计时安培法

Abstract:

A carbon paste electrode used as the working electrode, the electrochemical behavior of erythromycin[JP] ethylsuccinate(EEs) was investigated by cyclic voltammetry(CV) and differential pulse voltammetry(DPV), a novel method was proposed for the determination of EEs. The results showed two irreversible anodic peaks appeared at 0.83 V and 0.97 V, respectively in 0.1 mol/L PBS buffer solution(pH=8.0), indicating that there were two-step irreversible oxidations. Quantitative measurements by chronoamperometry(I-t) showed that the peak currents were proportional to erythromycin ethylsuccinate concentration in the range of 2.0×10-7~2.8×10-6 and 2.8×10-6~3.1×10-5 mol/L, respectively, with the detection limit(S/N=3) of 1.0×10-7 mol/L. The recovery was in the range of 95.0%～98.8% with a RSD of 3.4%(n=3). The method showed a high selectivity and sensitivity, and could be used for the determination of EEs in the drugs with satisfactory result.

Key words: erythromycin ethylsuccinate, carbon paste electrode, electrochemical determination, chronoamperometry (I-t)

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