pH和温度敏感型聚合物荧光探针的合成及应用

doi:10.11944/j.issn.1000-0518.2018.04.170463

摘要/Abstract

摘要：

以萘酰亚胺(NI)、N-异丙基丙烯酰胺(NIPAM)和多面体齐聚倍半硅氧烷(POSS)为原料,通过可逆加成断裂自由基聚合(RAFT)方法,设计合成了具有pH响应和温度敏感的聚合物荧光探针Poly(POSS-NI-NIPAM)。通过核磁共振波谱仪(NMR)、荧光发射光谱仪和质谱仪(MS)等技术手段表征了Poly(POSS-NI-NIPAM)的pH和温度的响应性能。结果表明,在pH值5.8~8.0范围内,探针Poly(POSS-NI-NIPAM)的荧光强度变化明显,与pH值呈现良好的线性相关(λ_em=520 nm,pK_a=6.51),对pH和温度的响应均呈现出良好的循环检测性能,可用于检测活细胞HeLa的pH值变化。探针Poly(POSS-NI-NIPAM)在检测生物样本中pH/温度变化方面具有潜在的应用前景。

关键词: 聚合物荧光探针, pH敏感, 温度敏感, 多面体笼状硅氧烷

Abstract:

A pH and thermo-responsive fluorescent polymeric sensor Poly(POSS-NI-NIPAM) was synthesized from naphthalimide(NI), N-isopropyl acrylamide(NIPAM) and polyhedral oligomeric silsesquioxane(POSS) as raw materials by the reversible addition-fragmentation chain transfer polymerization(RAFT). The pH and thermo-responsive properties of Poly(POSS-NI-NIPAM) were characterized by nuclear magnetic resonance spectroscopy(NMR), fluorescence emission spectrometer and mass spectrometry(MS). The results show that Poly(POSS-NI-NIPAM) shows obvious fluorescence changes in the range of pH 5.8~8.0 with a reversible performance. The high sensitivity for pH/temperature makes Poly(POSS-NI-NIPAM) as a promising fluorescent probe for the detection of intracellular pH/temperature in living cells.

Key words: polymeric fluorescent probe, pH sensitivity, temperature sensitivity, polyhedral oligomeric silsesquioxane

陈帆, 郭志前, 朱为宏. pH和温度敏感型聚合物荧光探针的合成及应用[J]. 应用化学, 2018, 35(4): 401-409.

CHEN Fan, GUO Zhiqian, ZHU Weihong. Synthesis and Characterization of pH and Temperature Sensitive Polymeric Fluorescent Probe[J]. Chinese Journal of Applied Chemistry, 2018, 35(4): 401-409.

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