应用化学 ›› 2018, Vol. 35 ›› Issue (4): 401-409.DOI: 10.11944/j.issn.1000-0518.2018.04.170463

• 研究论文 • 上一篇    下一篇

pH和温度敏感型聚合物荧光探针的合成及应用

陈帆,郭志前(),朱为宏   

  1. 华东理工大学精细化工研究所 上海 200237
  • 收稿日期:2017-12-21 接受日期:2017-01-24 出版日期:2018-03-30 发布日期:2018-04-02
  • 通讯作者: 郭志前
  • 基金资助:
    国家自然科学基金(21622602,21325625)项目资助

Synthesis and Characterization of pH and Temperature Sensitive Polymeric Fluorescent Probe

CHEN Fan,GUO Zhiqian(),ZHU Weihong   

  1. Institute of Fine Chemicals,East China University of Science & Technology,Shanghai 200237,China
  • Received:2017-12-21 Accepted:2017-01-24 Published:2018-03-30 Online:2018-04-02
  • Contact: GUO Zhiqian
  • Supported by:
    Supported by the National Natural Science Foundation of China(No.21622602, No.21325625),

摘要:

以萘酰亚胺(NI)、N-异丙基丙烯酰胺(NIPAM)和多面体齐聚倍半硅氧烷(POSS)为原料,通过可逆加成断裂自由基聚合(RAFT)方法,设计合成了具有pH响应和温度敏感的聚合物荧光探针Poly(POSS-NI-NIPAM)。 通过核磁共振波谱仪(NMR)、荧光发射光谱仪和质谱仪(MS)等技术手段表征了Poly(POSS-NI-NIPAM)的pH和温度的响应性能。 结果表明,在pH值5.8~8.0范围内,探针Poly(POSS-NI-NIPAM)的荧光强度变化明显,与pH值呈现良好的线性相关(λem=520 nm,pKa=6.51),对pH和温度的响应均呈现出良好的循环检测性能,可用于检测活细胞HeLa的pH值变化。 探针Poly(POSS-NI-NIPAM)在检测生物样本中pH/温度变化方面具有潜在的应用前景。

关键词: 聚合物荧光探针, pH敏感, 温度敏感, 多面体笼状硅氧烷

Abstract:

A pH and thermo-responsive fluorescent polymeric sensor Poly(POSS-NI-NIPAM) was synthesized from naphthalimide(NI), N-isopropyl acrylamide(NIPAM) and polyhedral oligomeric silsesquioxane(POSS) as raw materials by the reversible addition-fragmentation chain transfer polymerization(RAFT). The pH and thermo-responsive properties of Poly(POSS-NI-NIPAM) were characterized by nuclear magnetic resonance spectroscopy(NMR), fluorescence emission spectrometer and mass spectrometry(MS). The results show that Poly(POSS-NI-NIPAM) shows obvious fluorescence changes in the range of pH 5.8~8.0 with a reversible performance. The high sensitivity for pH/temperature makes Poly(POSS-NI-NIPAM) as a promising fluorescent probe for the detection of intracellular pH/temperature in living cells.

Key words: polymeric fluorescent probe, pH sensitivity, temperature sensitivity, polyhedral oligomeric silsesquioxane