以烟草花叶病毒为半抗原载体的活体免疫反应

doi:10.11944/j.issn.1000-0518.2015.10.150116

摘要/Abstract

摘要：

以烟草花叶病毒的赖氨酸突变体(TMV-EPMK)为半抗原载体,利用“点击化学”反应,将半抗原小分子雌三醇(E3)经由两个不同亲/疏水性的连接臂修饰在TMV-EPMK上,成功制备了TMV-EPMK/E3完全抗原。通过免疫实验发现,以亲水性连接臂制备的TMV-EPMK/E3偶联体可在小鼠体内诱导更高水平的E3特异性免疫反应,所产生的IgG抗体的滴度达21800。该结果表明,以烟草花叶病毒为载体制备的完全抗原,其免疫原性受连接臂性质的影响,亲水性的连接臂更有利于抗体反应的发生。

关键词: TMV-EPMK, 点击化学, 半抗原, 人工抗原, 免疫反应

Abstract:

Using a lysine mutant of tobacco mosaic virus(TMV-EPMK) as the hapten carrier, artificial TMV-EPMK/E3 antigens were fabricated by conjugating a small molecular estriol(E3) onto TMV-EPMK through “click” chemistry, and their immune response was evaluated in BALB/c mice. The results demonstrated that TMV-EPMK/E3 antigen containing the hydrophilic linker could enhance the E3 specific immune response with the higher IgG antibody level(titer 21800) by displaying E3 on TMV surface with distinctive topology. This study investigated that immune response was affected by linker property using TMV as the hapten carrier. A hydrophilic linker could elicit a stronger immune response.

Key words: TMV-EPMK, click chemistry, hapten, artificial antigen, immune response

赵霞,陈利民,胡君,张晓磊,林园,王倩. 以烟草花叶病毒为半抗原载体的活体免疫反应[J]. 应用化学, 2015, 32(10): 1107-1113.

ZHAO Xia,CHEN Limin,HU Jun,ZHANG Xiaolei,LUCKANAGUL Jittima Amie,LIN Yuan,WANG Qian. In vivo Immune Response Using Tobacco Mosaic Virus as Hapten Carrier[J]. Chinese Journal of Applied Chemistry, 2015, 32(10): 1107-1113.

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