红树林植物尖瓣海莲内生真菌Penicillium sp.B21次级代谢产物的分离鉴定

doi:10.11944/j.issn.1000-0518.2015.10.150076

摘要/Abstract

摘要：

研究了红树林植物尖瓣海莲叶内生真菌Penicillium sp.B21次级代谢产物。用硅胶柱层析、制备薄层层析和重结晶等方法,从该菌发酵液的乙酸乙酯萃取相中首次分离获得5个单体化合物,运用现代波谱技术、单晶X射线衍射以及文献数据对照,鉴定其结构分别为8-羟基-6甲基-1-甲氧羰基酮(1)、大黄素(2)、ω-羟基大黄素(3)、(3R,4S)-6,8-二羟基-3,4,5-三甲基-3,4-二氢异香豆素(4)和3,6,8-三羟基-3,5,7-三甲基-3,4-二氢异香豆素(5)。首次获得了sclerotinin B单晶结构。

关键词: 红树林内生真菌, Penicillium sp.B21, 次级代谢产物, 分离鉴定, X射线单晶衍射

Abstract:

The secondary metabolites of the medicinal mangrove endophytic fungus Penicillium sp.B21 were investigated. Five compounds were firstly isolated from the ethyl acetate extract of the fermentation mixture using silica gel column chromatography, preparative thin-layer chromatography and recrystallization. The structures of these compounds were identified as janthinone(1), emodin(2), citreorosein(3), decarboxydihydrocitrinone(4) and sclerotinin B(5), by comprehensive spectroscopic characterizations, X-ray single crystal diffraction as well as comparison with reported data. The single crystal structure of compound 5 was firstly obtained.

Key words: mangrove endophytic fungus, Penicillium sp. B21, secondary metabolites, isolation and elucidation, X-ray single crystal diffraction

罗由萍,邓鹏飞,牛燕燕,郑彩娟,闫向阳,陈军. 红树林植物尖瓣海莲内生真菌Penicillium sp.B21次级代谢产物的分离鉴定[J]. 应用化学, 2015, 32(10): 1196-1200.

LUO Youping,DENG Pengfei,NIU Yanyan,ZHENG Caijuan,YAN Xiangyang,CHEN Jun. Isolation and Structure Elucidation of Secondary Metabolites from Mangrove Endophytic Fungus Penicillium sp.B21[J]. Chinese Journal of Applied Chemistry, 2015, 32(10): 1196-1200.

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