Cell Biocompatibility of Photodeformable Azobenzene⁃containing Liquid Crystal Polymers

doi:10.19894/j.issn.1000-0518.210380

Chinese Journal of Applied Chemistry ›› 2021, Vol. 38 ›› Issue (10): 1371-1381.DOI: 10.19894/j.issn.1000-0518.210380

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Cell Biocompatibility of Photodeformable Azobenzene⁃containing Liquid Crystal Polymers

Sha-Man LUO¹, Hao-Zhe SUN², Shi-Qiang YAN¹, Hui HUANG¹, Wei-Jia ZHANG¹, Jia WEI², Yan-Lei YU²()

¹Institutes of Biomedical Sciences，Fudan University，Shanghai 200433，China
²Department of Materials Science，Fudan University，Shanghai 200433，China
³State Key Laboratory of Molecular Engineering of Polymers, Fudan University，Shanghai 200433, China

Received:2021-07-30 Accepted:2021-09-13 Published:2021-10-01 Online:2021-10-15
Contact: Yan-Lei YU
About author:ylyu@fudan.edu.cn
Supported by:
National Natural Science Foundation of China(51927805);China Postdoctoral Science Foundation(2019M651382)

Abstract

Abstract:

The cell biocompatibility of photodeformable azobenzene-containing liquid crystal polymer （PC11AB6） was investigated and compared with other common materials （polystyrene， glass and polydimethylsiloxane） in this study. After modifying surfaces with plasma treatment and coating， there was no significant difference in spreading state of Madin-Darby canine kidney （MDCK） cells， human umbilical vein endothelial cells （HUVEC） and induced pluripotent stem cells （iPSC） on the four materials； The expression of endothelial cell marker VWF in HUVEC and the expression of stem cell markers SOX2 and OCT3 in iPSC cultured on polymer PC11AB6 film were not significantly different from those of the other three materials； The growth and proliferation of HUVEC on the PC11AB6 film were comparable to those on polystyrene or glass， and were significantly better than those of polydimethylsiloxane. Overall， photodeformable azobenzene-containing LCP PC11AB6 is cell biocompatible， and shows potential in controllable microfluid transportation， micro biochemical analysis and the laboratory-on-chip system.

Key words: Photodeformable liquid crystal polymer, Azobenzene, Cell biocompatibility

CLC Number:

O631

Sha-Man LUO, Hao-Zhe SUN, Shi-Qiang YAN, Hui HUANG, Wei-Jia ZHANG, Jia WEI, Yan-Lei YU. Cell Biocompatibility of Photodeformable Azobenzene⁃containing Liquid Crystal Polymers[J]. Chinese Journal of Applied Chemistry, 2021, 38(10): 1371-1381.

Figures/Tables 10

Fig.1 （A） Chemical structure，（B） DSC curves of PC11AB6 upon heating and cooling and （C） POM of the liquid crystal polymer PC11AB6 during heating and cooling（ the scale bar is 50 μm）

Fig.2 SEM images of surface morphology of PC11AB6 polymer films， PS， Glass and PDMS

Fig.3 Static contact angles of water on PC11AB6 surfaces （A） before and （B） after plasma treatment

Fig.4 （A） Ultraviolet-Visible absorption spectrum and （B） fluorescence spectra of PC11AB6 polymer solution in dichloromethane

Fig.5 （A）Hematoxylin-Eosin staining of MDCK， HUVEC and iPSC cells and（B）F-actin immunodetection of MDCK and HUVEC cells on surfaces

Fig.6 Mean gray value of F-actin in MDCK and HUVEC on surfaces （n=3）

Fig.7 Immunodetection of （A） endothelial marker CD31 and VWF in HUVEC and （B—D）stem cell marker NANOG， SOX2 and OCT3 in iPSC on surfaces

Fig.8 Mean gray value of CD31 and VWF in HUVEC on surfaces （n=3）

Fig.9 Mean gray value of NANOG， SOX2 and OCT3 in iPSC on surfaces （n=3）

Fig.10 Cell proliferation of HUVEC on surfaces （OD values of formazan formation by viable cells were normalized by area of each type of surfaces）

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Cell Biocompatibility of Photodeformable Azobenzene⁃containing Liquid Crystal Polymers

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