Enzymatic Synthesis of Rubusoside and Its Inhibition on Liver and Gastrointestinal Cells

doi:10.11944/j.issn.1000-0518.2020.07.200020

Chinese Journal of Applied Chemistry ›› 2020, Vol. 37 ›› Issue (7): 785-792.DOI: 10.11944/j.issn.1000-0518.2020.07.200020

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Enzymatic Synthesis of Rubusoside and Its Inhibition on Liver and Gastrointestinal Cells

ZHOU Zhuoyu^a,b, SUI Xiaochen^a,b, XIA Yongmei^a,b,*

^aSchool of Chemical and Materials Engineering Jiangnan University,Wuxi,Jiangsu 214000,China;
^bState Key Laboratory of Food Science and Technology,Wuxi,Jiangsu 214000,China

Published:2020-07-01 Online:2020-07-07
Contact: XIA Yongmei, professor; Tel:0510-85329291; Fax:0510-85919625; E-mail:ymxia@jiangnan.edu.cn; Research interests:enzyme engineering, synthesis and application of fine chemicals
Supported by:
Supported by the National Natural Science Foundation of China(No.31772017, No.31371837.), the National Industry Technology and Engineering Double First-Class Discipline Program(No.LITE2018-03)

Abstract

Abstract: Rubusoside is a rare natural sweetener. In this experiment, a β-glucosidase from Aspergillus niger was screened from some glycosidases, which was used to proficiently produce rubusoside from stevioside. The optimum rubusoside yield and the stevioside conversion reach 90.4% and 98.8% in 12 h, respectively. Subsequently, the cytotoxicity of rubusoside on human gastrointestinal and liver cells was investigated, which includes 3 normal and 11 cancer cell lines. At a mass concentration of 250 μg/mL, rubusoside is not toxic to the normal cells, and its inhibition rate of rubusoside on BEL-7404 is 30% of that 5-fluorouracil proceeded.

Key words: rubusoside, steviolside, β-glucosidase, enzyme catalysis, anti-cancer

ZHOU Zhuoyu, SUI Xiaochen, XIA Yongmei. Enzymatic Synthesis of Rubusoside and Its Inhibition on Liver and Gastrointestinal Cells[J]. Chinese Journal of Applied Chemistry, 2020, 37(7): 785-792.

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Enzymatic Synthesis of Rubusoside and Its Inhibition on Liver and Gastrointestinal Cells

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