Abstract:

Fifty pesticides in Angelica sinensis, were determined by ultra-high performance liquid chromatography/tandem mass spectrometry(UPLC-MS/MS). Through comparing 11 kinds of purification methods, the best effective matrix purification method was selected. The pesticide residues were extracted from samples with acetoniteile, cleaned-up with NaCl and then analyzed using UPLC-MS/MS in dynamic multiple reaction monitoring(dMRM) mode with positive and negative electrospray ionization. The pesticide residues were quantified by matrix matched standard solution-internal standard method. All calibration curves showed good linearity(R²>0.99) within the test ranges. The average recoveries of most pesticides at the spiked levels of 10, 50, 100 μg/kg ranged from 70.1%~117.7% with RSDs of 1.0%~20.0%. The limits of quantification of 50 pesticides were 1.0~20.0 μg/kg. The method provides a reliable basis for accurate, efficient and economical detection of targeted analytes in Angelica sinensis.

Key words: matrix purification method, pesticide residues, Angelica sinensis