Hydrogen Peroxide and Glucose Sensitive Colorimetric Method Based on Deuterohemin-Ala His Thr Val Glu Lys

doi:10.11944/j.issn.1000-0518.2019.07.190047

Chinese Journal of Applied Chemistry ›› 2019, Vol. 36 ›› Issue (7): 847-854.DOI: 10.11944/j.issn.1000-0518.2019.07.190047

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Hydrogen Peroxide and Glucose Sensitive Colorimetric Method Based on Deuterohemin-Ala His Thr Val Glu Lys

Xin SHI,Chuanzhi LIU,Ping GONG,wei LI,Yue HOU()

School of Life Science and Technology,Changchun University of Science and Technology,Changchun 130022,China

Received:2019-02-21 Accepted:2019-04-30 Published:2019-07-01 Online:2019-07-02
Contact: Yue HOU
Supported by:
Supported by the Scientific and Technological Research Project Foundation of Jilin Provincial Science and Technology Department(No.20170204029SF)

Abstract

Abstract:

A simple colorimetric method for determination of H₂O₂ and glucose was established based on the peroxidase-like activity of deuterohemin-Ala His Thr Val Glu Lys(DhHP-6) to catalyze the oxidation of 4-aminoantipyrine(4-APP)-chlorophenol color reaction system in the presence of H₂O₂. The effects of pH, substrate concentration and DhHP-6 concentration were observed, and the reaction linearity, stability, correlation and recovery rate of the colorimetric method were detected. Under optimal reaction conditions, the activity of DhHP-6 is superior to that of peroxidase(POD) under different temperatures and times; the Michaelis constant(K_m) and maximum reaction rate(v_max) of DhHP-6 catalyzing H₂O₂ are 0.171 mmol/L and 4.22×10^-6 mol/s, respectively; the linearity range of H₂O₂ response is 0.39~25.0 mmol/L, the coefficient of variation(CV) and spiked recovery of the high, medium and low levels are between 1.29%~2.16% and 94.5%~101.1%, respectively; and correlation coefficient with the commercial kit is 0.9946; the concentration of glucose in 36 blood samples are 4.26~17.48 mmol/L. There is no significant difference between the two sets of data for glucose assay kit(P>0.05). Therefore, this is a simple, cheap, convenient and sensitive colorimetric determination method.

Key words: hydrogen peroxide, deuterohemin-Ala His Thr Val Glu Lys, mimetic enzyme, colorimetric analysis

Xin SHI,Chuanzhi LIU,Ping GONG,wei LI,Yue HOU. Hydrogen Peroxide and Glucose Sensitive Colorimetric Method Based on Deuterohemin-Ala His Thr Val Glu Lys[J]. Chinese Journal of Applied Chemistry, 2019, 36(7): 847-854.

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Hydrogen Peroxide and Glucose Sensitive Colorimetric Method Based on Deuterohemin-Ala His Thr Val Glu Lys

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