Chinese Journal of Applied Chemistry ›› 2018, Vol. 35 ›› Issue (12): 1411-1419.DOI: 10.11944/j.issn.1000-0518.2018.12.180186

• Review • Previous Articles     Next Articles

Application of Three-Dimensional Optical Tomography for in Vivo Bioimaging

LI Zhuhengab,ZHANG Huab,LIU Dianjunb,WANG Zhenxinb*()   

  1. aJilin Provincial Institute of Education,Changchun 130024,China
    bState Key Laboratory of Electroanalytical Chemistry,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,China
  • Received:2018-05-23 Accepted:2018-08-14 Published:2018-12-05 Online:2018-12-05
  • Contact: WANG Zhenxin
  • Supported by:
    Supported by the National Natural Science Foundation of China(No.201475126)


During three dimensional optical tomography bioimaging, the imaging targets(e.g., cells or tissues) were labeled by optical probes that can emit luminescence or generate strong light scattering under the excitation of an external light source. The three dimensional bioimage including the distribution of emission light in tissue and optical parameters of tissues can be reconstructed by combination of light intensity at the tissue boundary and the model of the photon propagating in the tissue. Three-dimensional optical tomography can provide the distribution information of target in the organism, overcome the limitation of plane imaging, and have great potential in tumor detection, gene expression, protein molecular detection, and revealing the change of body function. Through analyzing the recently published research results, the review summarizes the recent progress of three-dimensional optical tomography imaging technologies including optical coherence tomography(OCT), fluorescent molecular tomography(FMT), bioluminescence tomography(BLT) and Cerenkov luminescence tomography(CLT) in the biomedical and bioanalytical fields. The technical challenges faced in practical applications are analyzed, and the corresponding solutions are discussed.

Key words: optical coherence tomography, fluorescent molecular tomography, bioluminescence tomography, cerenkov luminescence tomography, multifunctional optical nanoprobe