Abstract:

For sensitive bioassay of hemoglobin(Hb), we synthesized Hb imprinted polymers(PIPs) on the surface of 3D Au/Cu nanodendrites prepared on Au electrode. The 3D Au/Cu nanodendrites were fabricated by electrodeposition performed by chronoamperometry at -0.9 V(vs.SCE) for 400 s in an aqueous electrolyte containing CuSO₄·5H₂O(0.1 mol/L) and HAuCl₄·4H₂O(0.025 mol/L). The PIPs was prepared with acrylamide as functional monomer, N,N'-methylenebisacrylamide and Hb as crosslinking agent and the template, respectively. The electrode modified with PIPs was examined by cyclic voltammetry(CV) and scanning electron microscope(SEM). Assays of Hb by differential pulse voltammetry(DPV) with PIPs modified electrode show broader linear range and lower detection limit for Hb determination compared to those similar sensors based on PIPs. The linear range is 1.0×10^-14~1.0×10^-1 mg/L with a detection limit at 1.9×10^-15 mg/L(S/N=3). These results indicate that the 3D Au/Cu nanodendrites greatly improve electron transfer ability of electrodes, and also improve the detection range of electrodes modified with PIPs.

Key words: three-dimensional nanodendrites, hemoglobin, protein imprinted polymers