Preparation and Characterization of Functional Tobacco Mosaic Virus Spherical Nanoparticles

doi:10.11944/j.issn.1000-0518.2017.04.160302

Chinese Journal of Applied Chemistry ›› 2017, Vol. 34 ›› Issue (4): 379-384.DOI: 10.11944/j.issn.1000-0518.2017.04.160302

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Preparation and Characterization of Functional Tobacco Mosaic Virus Spherical Nanoparticles

WU Yehong^a^b,ZHAO Xia^a,HU Jun^a,LIN Yuan^a^*(),WANG Qian^a^c

^aChangchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,China
^bUniversity of Chinese Academy of Sciences,Beijing 10049,China
^cUniversity of South Carolina,South Carolina 29208,USA

Received:2016-07-28 Accepted:2016-10-08 Published:2017-03-31 Online:2017-03-31
Contact: LIN Yuan
Supported by:
Supported by the National Natural Science Foundation of China(No.21429401, No.21374119), the State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry and the State Key Laboratory of Precision Measurement Technology and Instruments, Tianjin University

Abstract

Abstract:

Tobacco mosaic virus(TMV) due to its good biocompatibility, monodisperse, multivalent, low cost and other advantages, has been used as the basic building blocks used in optoelectronic devices, tissue engineering, vaccine vector, inorganic material preparation fields. However, compared to the rod like TMV, spherical TMV nanoparticles with no nucleic acid molecules are more resistant to environmental impact and have a larger specific surface area. The genetic and chemical modification of TMV, such as cysteine mutants of TMV(TMV TMV-Cys), lysine mutant of TMV(TMV-EPMK) and β-cyclodextrin(β-CD) modified TMV(TMV-β-CD) were conducted by thermal denaturation, and we studied their formation process and functionality. The results shows that the modified TMV can transit into the uniform spherical nanoparticles, and the functional groups exposed to the surface of the nanoparticles still keep their reactive ability.

Key words: tobacco mosaic viruses, thermal denaturation, spherical nanoparticles, functionality, mutant

WU Yehong, ZHAO Xia, HU Jun, LIN Yuan, WANG Qian. Preparation and Characterization of Functional Tobacco Mosaic Virus Spherical Nanoparticles[J]. Chinese Journal of Applied Chemistry, 2017, 34(4): 379-384.

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Preparation and Characterization of Functional Tobacco Mosaic Virus Spherical Nanoparticles

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