Chinese Journal of Applied Chemistry

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The Applications of Multivariate Curve Resolution-alternating Least Squares in Resolving Overlapped Spectra of Corydalis yanhusuo Fingerprint and the Content Detection of Tetrahydropalmatine

JIA Zehui1, WANG Chuntao1, LI Hua2*   

  1. (1.Department of Chemistry,Taiyuan Normal College,Taiyuan 030031,China;
    2.Institute of Analytical Science/Research Center for Modernization of
    Chinese Herbal Medicines,Northwest University,Xi′an 710069,China)
  • Received:2012-04-27 Revised:2012-06-25 Online:2013-03-10 Published:2013-03-10

Abstract: Spectral correlative chromatography was used to identify the peak of tetrahydropalmatine in Corydalis yanhusuo fingerprint. Moreover, multivariate curve resolution-alternating least squares(MCR-ALS) was used to resolve the overlapping peaks of tetrahydropalmatine and restore its true peak shape. More than 99.9% spectrum could be recovered and accurately quantitative results were obtained. The method can be used as an auxillary fingerprinting method to provide a reliable basis for the authenticity and quality assessment of Chinese medicines. The effect of the herbs-cooking on the content of tetrahydropalmatine was also discussed.

Key words: corydalis yanhusuo, fingerprint of traditional Chinese medicine, Spectral correlative chromatography, Multivariate curve resolution-alternating least squares

CLC Number: