Abstract: The surface-enhanced NIR-FT-Raman spectra of two vitamin B drugs in-situ recorded from thin layer chromatographic(TLC) plate are reported. The in-situ identification of TLC spots from 3 μg-drug reveals similar main spectral feature as that of solid samples. In addition, the sensitivity enhancement has been improved greatly by using silver colloid activated thin layer chromatographic spots.

Key words: vitamin B₁, vitamin B₂, thin layer chromatography, SERS, NIR-FT-Raman