Abstract: An enzyme microeleetrode for glucose determination has been prepared from glucose oxidase and dimerhylferrocene (DMFc) immobilized in Eastman-AQ film on microdisk array of carbon fibers.The ferrocene derivative couple(Fc/Fc⁺) plays the role of mediator for electron transfer between immobilized glucose oxidase and the electrode. A linear current response to the glucose concentration over a range of 1～12mmol/L was observed at this en zyme microelectrode. The influences of pH and temperature on the enzyme electrode behavior are also presented. The response times was found to be less than 15s. The interference of the electroactive species such as ascorbic and uric acid could be effectively depressed. The resuits of analysis of serum samples were in good agreement with those obtained by photometric analysis.

Key words: glucose biosensor, Eastman-AQ polymer, dimethyl ferrocene, microdisk array electrode