Abstract:

Enantiomers of amino acids and chiral drugs were separated by capillary zone electrophoresis using double (6-O-β-carboxy-methyl-1,4-maleic acid ester)-β-cyclodextrin(DOCB-β-CD) as chiral selector. The buffer solution used was 20 mmol/L phosphate. The influences of DOCB-β-CD concentration, background buffer pH value, temperature and applied voltage were investigated. Under the respective optimum condition, enantiomers separation of four kinds of DL-amino acids(Phenylalanine, Tryptophan, Tyrosine and Histidine) and two kinds of chiral drugs(rofecoxib and rosiglitazone) were attained with a clear separation from the baseline.

Key words: Capillary electrophoresis, β-cyclodextrin derivative, DL-amino acid, chiral drugs