Abstract:

Supercritical CO2 fluid(SFE-CO2) extraction technique was used to extract the rare ginsenosides in processed ginseng, and extracting productions were analyzed by HPLC/ESI-MSn. The optimal extraction conditions were estimated as:the extraction pressure was 35 MPa, the extraction temperature was at 50 ℃， and the concentration of alcohol as entrainer was 70%. The experimental result showed that the extracting efficiency of SFE-CO2 technique is similar with that of transonic extraction. The extracting method using SFE-CO2 technique is in favor of ambient.

Key words: rare Ginsenosides, supercritical CO2 fluid extraction, analyzed by HPLC-ESI-MS