明胶改性聚醚醚酮微载体的制备、表征及性能

doi:10.19894/j.issn.1000-0518.210040

摘要/Abstract

摘要： 微载体因其具有较高的表面积/体积比等优点可以大大提高哺乳动物细胞培养效率,被广泛应用于生物制药和组织工程等领域。但微载体多为一次性使用,不耐高温,且主要依赖进口,价格昂贵,因而限制了其国内的应用和推广。聚醚醚酮(PEEK)材料具有良好的生物相容性、化学稳定性及耐高温等特性,是一种优异的微载体材料,但存在熔点高,加工方法单一和生物惰性等缺陷。本文以浓硫酸为溶剂,乙醇溶液为萃取剂,采用气流辅助滴注/相分离法,将PEEK制备成448 μm左右,尺寸均匀的微球;经氨丙基三乙氧基硅烷(APTES)处理,获得表面氨基化修饰的PEEK微球(PEEK-N);进一步,以N,N'-羰基二咪唑(CDI)为活性中间体,将明胶分子接枝到PEEK-N微球表面,获得表面明胶修饰的PEEK微载体(PEEK-G)。对材料的物理化学性质、表面接枝量进行表征;并通过体外细胞实验评估其细胞毒性、细胞粘附效率和细胞增殖能力。结果显示,通过该方法制备成功了3种不同明胶接枝含量的PEEK细胞微载体(PEEK-G1,PEEK-G2,PEEK-G3),其中明胶含量较高的PEEK-G3毒性最低,细胞粘附和增殖效果最理想。

关键词: 微载体, 聚醚醚酮, 明胶修饰, 小鼠胚胎成骨细胞前体细胞, 细胞增殖与粘附

Abstract: Due to its high specific surface area which can greatly improve the efficiency of cell culture, the microcarrier is widely used in the fields of biopharmaceutical and tissue engineering. However, the cost is too high because most of the microcarriers are disposable and imported. This situation has limited the application of microcarrier in domestic. The polyetheretherketone (PEEK) as a desired material for preparing microcarriers has many properties such as good biocompatibility, chemical stability and high temperature resistance. But it is hard to process PEEK into microcarrier. In this paper, in order to prepare the microcarrier through airflow assisted infusion/phase separation method, the PEEK powder was dissolved in concentrated sulfuric acid, followed by dripping into H₂O/ethanol solution. The diameter of the uniform microcarriers is near 448 μm. Furthermore, the amination reaction on microcarriers surface was governed by treated with aminopropyltriethoxysilane (APTES). Then, the gelatin was grafted in different amounts on microcarriers using N,N'-carbonyldiimidazole (CDI) as reactive intermediate. At last, the cytotoxicity of microcarrier, the proliferation and adhesion of cell on microcarriers were evaluated. The results indicate that the high-gelatin-grafted microcarrier (PEEK-G3) is promised in enhancing cell proliferation and adhesion.

Key words: Microcarrier, Polyetheretherketone, Gelatin modification, MC3T3-E1, Cell proliferation and adhesion

中图分类号:

O631

焦自学, 高承哲, 孙硕, 武振旭, 章培标. 明胶改性聚醚醚酮微载体的制备、表征及性能[J]. 应用化学, 2021, 38(4): 390-397.

JIAO Zi-Xue, GAO Cheng-Zhe, SUN Shuo, WU Zhen-Xu, ZHANG Pei-Biao. Preparation, Characterization and Performance of Gelatin Modified Polyetheretherketone Microcarriers[J]. Chinese Journal of Applied Chemistry, 2021, 38(4): 390-397.

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