甜茶苷的酶法制备及其对肝肠胃细胞的抑制作用

doi:10.11944/j.issn.1000-0518.2020.07.200020

摘要/Abstract

摘要： 甜茶苷是一种自然界中含量稀少的甜味剂。本实验从多种糖苷酶中筛选出一种来自Aspergillus niger的β-葡萄糖苷酶,用以特异性水解斯替夫苷而高通量制备甜茶苷。最适条件下反应12 h后,甜茶苷的产率为90.4%,斯替夫苷的转化率达98.8%。为了进一步考察甜茶苷的安全性并拓展其潜在的用途,实验考察了甜茶苷对人胃肠道和肝脏细胞的细胞毒性,即甜茶苷对3种正常细胞以及11种癌细胞生长的抑制效果。实验结果表明,在250 μg/mL的质量浓度下,甜茶苷对正常人胃肠肝细胞无毒性;相同质量浓度下,甜茶苷对人体肝癌细胞BEL-7404的抑制率是5-氟尿嘧啶的30％。

关键词: 甜茶苷, 斯替夫苷, β-葡萄糖苷酶, 酶催化, 抑癌

Abstract: Rubusoside is a rare natural sweetener. In this experiment, a β-glucosidase from Aspergillus niger was screened from some glycosidases, which was used to proficiently produce rubusoside from stevioside. The optimum rubusoside yield and the stevioside conversion reach 90.4% and 98.8% in 12 h, respectively. Subsequently, the cytotoxicity of rubusoside on human gastrointestinal and liver cells was investigated, which includes 3 normal and 11 cancer cell lines. At a mass concentration of 250 μg/mL, rubusoside is not toxic to the normal cells, and its inhibition rate of rubusoside on BEL-7404 is 30% of that 5-fluorouracil proceeded.

Key words: rubusoside, steviolside, β-glucosidase, enzyme catalysis, anti-cancer

周卓愉, 隋晓辰, 夏咏梅. 甜茶苷的酶法制备及其对肝肠胃细胞的抑制作用[J]. 应用化学, 2020, 37(7): 785-792.

ZHOU Zhuoyu, SUI Xiaochen, XIA Yongmei. Enzymatic Synthesis of Rubusoside and Its Inhibition on Liver and Gastrointestinal Cells[J]. Chinese Journal of Applied Chemistry, 2020, 37(7): 785-792.

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