中华医学超声杂志

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上转换纳米粒子结合DNA链式扩增技术在阿霉素光控释放中的应用

于畅ab,邵帅ac,李贺杰d,刘国锋ae,丁彬彬af,马平安a*(),林君a*()   

  1. a中国科学院长春应用化学研究所,稀土资源利用国家重点实验室 长春 130022
    b中国科学院大学 北京 100049
    c长春理工大学化学与环境工程学院 长春 130022
    d吉林大学第二医院,耳鼻喉-头颈外科 长春 130041
    e吉林大学物理学院 长春 130012
    f中国科学技术大学 合肥 230026
  • 收稿日期:2018-03-09 出版日期:2018-12-05
  • 通讯作者: 马平安,林君
  • 基金资助:
    国家自然科学基金(51672268,51472233,51332008,51720105015,51628201,21521092),中国科学院前沿科学重点研究项目(YZDY-SSW-JSC018),吉林省科技引导计划(20170101188JC,20170414003GH)项目资助

Upconversion Nanoparticles Decorated with Hybridization Chain Reaction DNA for Near-infrared Light Controlled Release Doxorubicin

YU Changab,SHAO Shuaiac,LI Hejied,LIU Guofengae,DING Binbinaf,MA Ping'ana*(),LIN Juna*()   

  1. aState Key Laboratory of Rare Earth Resource Utilization,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,China
    bUniversity of Chinese Academy of Sciences,Beijing 100049,China
    cSchool of Chemistry and Environmental Engineering, Changchun University of Science and Technology,Changchun 130022,China
    dDepartment of Otorhinolaryngology Head and Neck Surgery, Second Hospital of Jilin University,Changchun 130041,China
    eCollege of Physics, Jilin University,Changchun 130012,China
    fUniversity of Science and Technology of China,Hefei 230026,China
  • Received:2018-03-09 Published:2018-12-05
  • Contact: MA Ping'an,LIN Jun
  • Supported by:
    Supported by the National Natural Science Foundation of China(No.NSFC51672268, No.51472233, No.51332008, No.51720105015, No.51628201, No.21521092), the Key Research Program of Frontier Sciences, CAS(No.YZDY-SSW-JSC018), Projects for Science and Technology Development Plan of Jilin Province(No.20170101188JC, No.20170414003GH)

被引次数

1

摘要：

纳米技术的发展使得纳米材料可以通过不同的表面包覆和修饰而在生物医药中发挥应用。 构建简单、经济、药物释放可控的生物相容性纳米药物仍是纳米生物化学领域的重点。 我们构建的纳米载药体系(DDS)以NaYF4：Yb/Tm上转换纳米粒子为载体,在其表面通过光致断键型小分子4,5-二甲氧基-2-硝基苯基乙酮(DMNPE)连接一段短单链DNA,利用DNA链式扩增技术(HCR)来调节纳米粒子最终修饰的双链DNA的总量,从而控制对抗癌药物阿霉素(Dox)的担载量,在980 nm激光照射下上转换纳米粒子发射可切断DMNPE连接的近紫外光,协同胞内DNA酶的作用达到对药物的可控释放。 由于近红外光照对生物组织具有较好的穿透能力,此体系能够对病灶位置有更好的光靶向性从而减少药物的毒副作用。

关键词: 上转换纳米粒子, DNA链式扩增, 控制释放, 盐酸阿霉素

Abstract:

Nanotechnology has allowed various coatings and modifications to nanostructures for applications of biomedicine. The constructions of simple, economical, controllable and biocompatible drug delivery systems(DDS) are still in demand. Here, we report a NaYF4：Yb/Tm nanoparticle-based DDS that anticancer drug doxorubicin(Dox) could be quantitatively carried by double stranded DNA thereon that self-assembled through a hybridization chain reaction(HCR). The HCR DNA was absorbed on the surface of upconversion nanoparticles(UCNPs) through linkage to photocleavable 1-(4,5-dimethoxy-2-nitrophenyl) diazoethane(DMNPE) molecules, which can be cleaved by near ultraviolet light emitted by UCNPs under 980 nm laser light excitation. The controllable release of model drug Dox by near-infrared(NIR) should facilitate selective cytotoxicity to target region and reduced side effects and could prove promising in biomedical applications.

Key words: upconversion nanoparticles, DNA hybridization chain reaction, controllable release, doxorubicin hydrochloride

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