共轭亚油酸与海藻酸钠囊泡化自组装纳米容器及其药物缓释性能

doi:10.11944/j.issn.1000-0518.2018.12.180046

摘要/Abstract

摘要：

脂肪酸囊泡(FAV)是一类重要的纳米容器,然而其形成pH范围较窄且偏碱性环境,限制了其应用。本文将共轭亚油酸(CLA)与海藻酸钠(SA)在近中性环境下共同自组装囊泡化纳米容器并提高其膜稳定性。动态激光光散射(DLS)和透射电子显微镜(TEM)结果表明,当SA质量分数为25%~50%时复合体系可在近中性条件下自组装形成50~250 nm尺寸的囊泡化纳米容器,且pH=7.4时随着质量分数增加囊泡化纳米容器直径增大。根据SA和CLA在中性环境的物种存在形式推测,二者通过氢键作用驱动形成囊泡化纳米容器。体外模拟释放实验表明,囊泡化纳米容器具有较高包覆率和较优缓释效果,有望应用于药物传输领域。

关键词: 纳米容器, 脂肪酸囊泡, 共轭亚油酸, 海藻酸钠, 药物缓释

Abstract:

Fatty acid vesicles(FAVs) are a kind of important nanocontainers, but they are inherently alkaline-adapted and the pH window for the FAV formation is very narrow, which hinders their application in drug delivery. In this work, the vesicular nanocontainers were fabricated by self-assembly of conjugated linoleic acid(CLA) and sodium alginate(SA) in neutral pH range, and the membrane stability of the FAVs was enhanced. Dynamic light scattering(DLS) and transmission electron microscopy(TEM) results indicated that the vesicular nanocontainers with vesicular diameter of 50~250 nm were self-assembled in neutral pH range when the mass fraction of SA was 25%~50%. Moreover, the size of the vesicular nanocontainers increased with the increase of mass fraction of SA at pH=7.4. Based on the chemical species of SA and CLA in neutral pH range, it was proposed that hydrogen bonding is the driving force to self-assemble them into the vesicular nanocontainers. In vitro drug release results showed that the vesicular nanocontainers had a higher encapsulation efficiency and better sustained release effect. The vesicular nanocontainers are expected to be promising carriers in drug delivery.

Key words: nanocontainer, fatty acid vesicle, conjugated linoleic acid, sodium alginate, drug delivery

樊晔, 韩贻陈, 夏咏梅, 薄纯玲, 王淑钰, 方云. 共轭亚油酸与海藻酸钠囊泡化自组装纳米容器及其药物缓释性能[J]. 应用化学, 2018, 35(12): 1478-1484.

FAN Ye, HAN Yichen, XIA Yongmei, BO Chunling, WANG Shuyu, FANG Yun. Investigation on Self-assembly of Nanocontainers by Vesiculation of Conjugated Linoleic Acid and Sodium Alginate and Their Drug Delivery Behavior[J]. Chinese Journal of Applied Chemistry, 2018, 35(12): 1478-1484.

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