Ag(Ⅲ)-聚六亚甲基双胍配合物的合成及抗菌性能表征

doi:10.11944/j.issn.1000-0518.2017.07.160443

摘要/Abstract

摘要：

采用水溶液反应法,以不同粘均相对分子质量的聚六亚甲基双胍(PHMB)作为配体,合成出一系列稳定的由400 nm左右的小颗粒团聚而来的微米级四配位Ag(Ⅲ)-PHMB配合物,并对其进行表征证明了物质的结构与价态。通过抗菌活性实验发现,Ag(Ⅲ)-PHMB配合物的抗菌活性随着配体PHMB的粘均相对分子质量的升高而增大,随着粒径增大而减小。在低浓度条件下,微米级Ag(Ⅲ)-PHMB配合物的抗菌性能高于同等质量浓度的AgO、AgNO₃与PHMB;随着质量浓度的升高,微米级Ag(Ⅲ)-PHMB配合物与细菌的实际接触面积、缓释出的抗菌活性物质增长趋缓,因而抗菌活性升高趋缓。

关键词: Ag(Ⅲ)-聚六亚甲基双胍配合物, 粘均相对分子质量, 抗菌性能

Abstract:

As the ligand and biological acceptable parts, polyhexamethylene biguanide hydrochloride(PHMB) are used for stabilizing Ag³⁺. A series of micron-sized quadricovalent cationic Ag(Ⅲ)-PHMB complexes was synthesized via oxidation of the monovalent silver, followed by stabilization of the oxidized high valence silver ions through complexation with PHMB. From the experimental result of agar well-diffusion method, it can be concluded that the antibacterial activity of Ag(Ⅲ)-PHMB complex is enhanced with increasing viscosity-average relative molecular mass of PHMB and weakened with the increase of the size of Ag(Ⅲ)-PHMB complex. The experimental results reveal that the complex yields the maximum antibacterial activity when the viscosity-average relative molecular mass of ligand is 5428. Though broth dilution method, micron-sized Ag(Ⅲ)-PHMB complex displays excellent antibacterial performance in low mass concentration towards Gram-positive and Gram-negative prokaryotes for excellent antibacterial activity of Ag³⁺. Compared with AgO, AgNO₃ and PHMB, Ag(Ⅲ)-PHMB complex has lower minimal inhibitory concentration(MIC). With the increase of mass concentration, however, because of the reduction of the growth ratio of contacting area between Ag(Ⅲ)-PHMB complex and bacteria and the release radio of antimicrobial active substance, antibacterial activity of micron-sized Ag(Ⅲ)-PHMB complex shows slowly increasing rate.

Key words: Ag(Ⅲ)-polyhexamethylene biguanide hydrochloride complex, viscosity-average relative molecular mass, antibacterial performance

赵鑫钰, 陈迅, 陆梓仪, 陈雷光, 朱贤锋, 侯琳熙. Ag(Ⅲ)-聚六亚甲基双胍配合物的合成及抗菌性能表征[J]. 应用化学, 2017, 34(7): 749-756.

ZHAO Xinyu, CHEN Xun, LU Ziyi, CHEN Leiguang, ZHU Xianfeng, HOU Linxi. Preparation and Antibiosis Characterization of Trivalent Ag(Ⅲ)-Polyhexamethylene Biguanide Hydrochloride Complex[J]. Chinese Journal of Applied Chemistry, 2017, 34(7): 749-756.

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