中华医学超声杂志

应用化学 ›› 1998, Vol. 0 ›› Issue (6): 25-28.

• 研究论文 • 上一篇    下一篇

功能聚间苯二胺膜用于免疫传感器分析

陈瑞川1, 卢方1, 李东辉1, 张长弓1, 万桢2   

  1. 1. 厦门大学抗癌研究中心 厦门361005;
    2. 厦门大学化学系 厦门
  • 收稿日期:1998-02-23 修回日期:1998-09-09 出版日期:1998-12-10 发布日期:1998-12-10

Application of Functional Electropolymerized m-Phenylene Diamine Membrane in Immunosensor Analysis

Chen Ruichuan1, Lu Fang1, Li Donghui1, Zhang Changgong1, Wan Zhen2   

  1. 1. Biochemistry Laboratory of Cancer Research Center, Xiamen University, Xiamen 361005;
    2. Department of Chemistry, Xiamen University, Xiamen
  • Received:1998-02-23 Revised:1998-09-09 Published:1998-12-10 Online:1998-12-10

PDF

350

被引次数

4 | 3

摘要： 以电化学聚合法在石墨电极上获得聚间苯二胺膜(PMPD),并建立了快速、灵敏的膜质量检验方法：辣根过氧化物酶(HRP)标记PMPD膜-邻苯二胺(OPD)反应法,对聚合、活化等条件作了研究；进一步制成了检测乙肝表面抗原(HBsAg)和检测鼠免疫蛋白(MIgG)的免疫电极,对血清样品作了检测。结果表明：2.5V电池电压下,于含0.12mol/LMPD的1.2mol/LH2SO4中聚合20min,再以30g/L浓度的戊二醛活化4h,所得HRP标记电极及免疫电极重现性好；检测HB-sAg和MIgG的线性范围分别为0.1～3.2mg/L及0.1～10mg/L.

关键词: 免疫传感器, 化学修饰电极, 聚间苯二胺

Abstract: An electropolymerization method of m-phenylene diamine membrane, polyphenylene diamine(PMPD) on the surface of graphite electrode is described. To identify the quality of the membrane, a rapid and sensitive method has been established based on the reaction of a horse-radish peroxidase(HRP) labeled PMPD with orthophenylene diamine. An anti-HBsAg and an antiMIgG immunoelectrodes were prepared for detecting hepatitis B surface antigen(HBsAg) and mouse immunoglobin G(MIgG). The optimum membrane preparation conditions are polymerization: 0.12 mol/L MPD solution in 1.2 mol/L H2SO4 for 20 min under 2.5 V, activation: with 3% glutiraldehyde for 4 h. The prepared HRP labeled electrode and immunoelectrodes possessed good repetition. Catibration graphs were linear for HBsAg in ranges from 0.132 g/mL and 0.120 g/mL for MIgG/mL.

Key words: immunosensor, chemically modified electrode, poly-m-phenylene diamine

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