应用化学 ›› 1998, Vol. 0 ›› Issue (1): 47-49.

• 研究论文 • 上一篇    下一篇

测定甲胎蛋白的非标记电位型免疫传感器

彭图治, 祝方猛, 杨丽菊, 何巧红, 程琼   

  1. 杭州大学化学系 杭州 310028
  • 收稿日期:1997-04-09 修回日期:1997-09-10 出版日期:1998-02-10 发布日期:1998-02-10
  • 基金资助:
    浙江省自然科学基金;分析测试基金

Potentiometric Determination of α-Fetoprotein (AFP) Using a Non-labeled Antibody Immunosensor

Peng Tuzhi, Zhu Fangmeng, Yang Liju, He Qiaohong, Cheng Qiong   

  1. Department of Chemistry, Hangzhou University, Hangzhou 310028
  • Received:1997-04-09 Revised:1997-09-10 Published:1998-02-10 Online:1998-02-10

摘要: 免疫分析是最重要的生物化学分析方法之一,是根据抗体与抗原或半抗原之间的专一性反应建立的分析方法,具有高度的选择性和极低的检测下限.电化学免疫传感器就是将免疫分析技术与电化学传感器相结合的一种新的免疫分析方法,具有免疫反应的高选择性和电化学分析的高灵敏性.

关键词: 甲胎蛋白, 电位法, 免疫传感器

Abstract: The anti-AFP antibody is immobilized on the surface of PVC-BSA(bovine serumalbumin) membrane, which has been activated by NH2OH·HCl solution.The immunosen-sor consists of such a membrane with added AFP and Ag-AgCl internal electrode.It showedlinear response over concentration range of 10-300μg/L and potential difference(△E) of 15~20 mV for 300μg/L of AFP.Other common antigens in human serum do not interfere withAFP's determination.

Key words: α-fetoprotein, potentiometry, immunosensor