应用化学 ›› 1997, Vol. 0 ›› Issue (5): 36-38.

• 研究论文 • 上一篇    下一篇

荧光法研究硒酶模型化合物消除·OH自由基的作用

黄祖云, 蔡汝秀, 肖颖歆, 刘秀芳, 徐汉生, 曾云鹗   

  1. 武汉大学化学系, 武汉 430072
  • 收稿日期:1996-12-25 修回日期:1997-07-14 出版日期:1997-10-10 发布日期:1997-10-10
  • 基金资助:
    国家自然科学基金

Studies on the Scavening Action to Hydroxyl Free Radical of Benzisoselenazolones and Diselenides by Fluorometry

Huang Zuyun, Cai Ruxiu, Xiao Yingxin, Lin Xiufang, Xu Hansheng, Zeng Yun'e   

  1. Department of Chemistry, Wuhan University, Wuhan 430072
  • Received:1996-12-25 Revised:1997-07-14 Published:1997-10-10 Online:1997-10-10

摘要: 利用Fenton反应产生的羟基自由基与苯甲酸生成具有荧光的羟化苯甲酸,用荧光法间接测定羟基自由基的含量。用荧光法研究新合成的5种硒酶模型化合物对羟基自由基的清除作用。结果表明,新合成的硒酶模型化合物对羟基自由基有良好的清除作用。该法具有简便、快速、灵敏等特点。

关键词: 荧光法, 羟基自由基, Fenton反应, 苯并异硒唑酮, 二硒醚

Abstract: The scavening action to hydroxyl free radical of benzisoselenazolones and diselenides has been studied by fluorometry. The hydroxyl free radicals were produced via Fenton reaction. The method used provides a sensitive, simple and accurate measurement of the scavening action to hydroxyl free radical for synthetic drugs of highly biological activity.

Key words: fluorometry, hydroxyl free radical, Fenton reaction, benzisoselenazolone, diselenide