中华医学超声杂志

应用化学 ›› 1992, Vol. 0 ›› Issue (1): 89-91.

• 研究简报 • 上一篇    下一篇

过氧化氢酶电极的研制及其性能

陆中庆, 周祖新, 曹大均, 章宗穰   

  1. 上海师范大学化学系, 上海 200234
  • 收稿日期:1990-11-27 修回日期:1991-04-05 出版日期:1992-02-10 发布日期:1992-02-10

CATALASE ENZYME ELECTRODE AND ITS PROPERTY

Lu Zhongqing, Zhou Zuxin, Cao Dajun, Zhang Zongrang   

  1. Department of Chemistry, Shanghai Teachers' University, Shanghai 200234
  • Received:1990-11-27 Revised:1991-04-05 Published:1992-02-10 Online:1992-02-10

PDF

415

被引次数

1

摘要： 生物传感器是一种新兴的测试工具,其中酶电极因具有较常规离子选择电极更短的响应时间,更好的选择性与抗干扰能力强等特性,引起了人们的关注。本文用新鲜合成的三乙基肟四氟化硼作为烷基化试剂,将过氧化氢酶(CTS)固定在尼龙网上,制成CTS酶电极,较Ciucu等[1]用2,4-二氯-6-甲氧基-S-三氮杂笨在透析膜上固定的方法简单,反应条件温和。测试了这种酶电极对H2O2:的响应性能。

关键词: 过氧化氢酶, 酶电极, 固定化, 过氧化氢测定

Abstract: A catalase membrane immobilized on a nylon net was prepared, using freshly synthesized triethylonium tetrafluoroborate as immobilizer. The membrane had an activity of 80unit/cm2,while that immobilized by dimethyl sulfate showed an activity only of 35, 6unit/cm2 .The catalase electrode showed linear response within 5, 0×10-6～5,0×10-4mol·L-1,with detection lirit of 4,0×10-6mol·L-1 and 95,2% recovery.Its service life is more than 500 times. SO42-, Cl-, NO3-,NH4+,Ac-,glycine,glueose etc. do not interfere with the detection.

Key words: catalase, enzyme electorde, immobilization, hydrogenperoxide determination

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