应用化学 ›› 2010, Vol. 27 ›› Issue (09): 1061-1064.DOI: 10.3724/SP.J.1095.2010.90853

• 研究论文 • 上一篇    下一篇

Oxazine750对平行四股螺旋结构的识别与稳定作用

陈名利,任劲松*   

  1. (中国科学院长春应用化学研究所 稀土资源与利用国家重点实验室 长春  130022)
  • 收稿日期:2009-12-18 修回日期:2010-01-28 出版日期:2010-09-10 发布日期:2010-09-10
  • 通讯作者: 任劲松,女,研究员; E-mail:jren@ciac.jl.cn; 研究方向:化学生物学
  • 基金资助:
    国家自然科学基金(20833006)资助项目

Parallel G-quadruplex Recognition and Stabilization by Oxazine750

CHEN Ming-Li, REN Jin-Song*   

  1. (Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022)
  • Received:2009-12-18 Revised:2010-01-28 Published:2010-09-10 Online:2010-09-10

摘要:

以d[TG4T]4、d[G4T4G4]2和c-myc序列为代表,采用竞争平衡透析、紫外光谱和圆二色光谱研究了oxazine750对四股螺旋结构的识别与稳定作用。 结果表明,oxazine750能够更好的结合在平行四股螺旋上,而对反平行四股螺旋结合较少。 热变性实验表明,oxazine750使d[TG4T]4形成的平行结构的四股螺旋的解旋温度提高了11 ℃,对c-myc形成的平行四股螺旋的解旋温度甚至提高了40 ℃。 而对d[G4T4G4]2形成的反平行结构解旋温度降低了近8 ℃。

关键词: 四股螺旋, 小分子, 竞争平衡透析

Abstract:

The bindings of small molecular oxazine750 to d[TG4T]4, d[G4T4G4]2 and c-myc sequences was characterized by means of circular dichroism, thermal denaturation, and so on. Oxazine750 was found to bind preferentially and strongly to parallel G-quadruplex. It can effectively stabilize the parallel G-quadruplex and destabilize the antiparallel G-quadruplex. The melting temperature of d[TG4T]4 or c-myc is increased by about 11 ℃ or 40 ℃ by oxazine750, respectively. Under the same condition, oxazine750 decreased the melting temperature of d[G4T4G4]2 by about 8 ℃.

Key words: G-quadruplex, small molecular, competition dialysis

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