中华医学超声杂志

应用化学 ›› 2010, Vol. 27 ›› Issue (06): 737-741.DOI: 10.3724/SP.J.1095.2010.90548

• 研究论文 • 上一篇    下一篇

基于与Ce3+相互作用共振光散射法测定三磷酸腺苷二钠

谭克俊*,马璐,覃明丽   

  1. (发光与实时分析教育部重点实验室，西南大学化学化工学院 重庆 400715)
  • 收稿日期:2009-08-24 修回日期:2009-10-16 出版日期:2010-06-10 发布日期:2010-06-10
  • 通讯作者: 谭克俊，男，博士，副教授; E-mail:tankj@swu.edu.cn; 研究方向：分析化学
  • 基金资助:
    国家自然科学基金（20877063）重庆市科学技术委员会基金（CSTC,2007BB7360）西南大学基金（SWUB2006007）资助项目

Determination of Adenosine Disodium Triphosphate by Resonance Light Scattering Technique Based on Its Interaction with Ce3+

TAN Ke-Jun*, MA Lu, QIN Ming-Li   

  1. (Education Ministry Key Laboratory on Luminescence and Real-Time Analysis,College of
    Chemistry and Chemical Engineering,Southwest University,Chongqing 400715)
  • Received:2009-08-24 Revised:2009-10-16 Published:2010-06-10 Online:2010-06-10

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摘要：

在Tris-HCl缓冲溶液中, 三磷酸腺苷二钠(ATP)与铈离子(Ce3+)相互作用产生很强的共振光散射(RLS)信号。实验表明，增强的散射信号与ATP的浓度在2.0~24 μmol/L范围内呈线性关系,据此建立了一种测定三磷酸腺苷的共振光散射法,检测限(S/N=3σ)为51.25 nmol/L。研究了共存物质的影响，用于临床三磷酸腺苷注射液的测定, RSD=2.29%。

关键词: 三磷酸腺苷二钠, 铈离子, 共振光散射

Abstract:

In Tris-HCl buffer solution, a resonance light scattering(RLS) detection method for adenosine disodium triphosphate(ATP) was developed based on RLS signal enhancement due to interactions of ATP with cerium ions in an alkalescent aqueous medium. The enhanced RLS intensity was proportional to ATP content in a range of 2.0~24 μmol/L with a limit of determination(LOD,S/N=3σ) of 51.25 nmol/L. The effects of coexisting substances were investigated. The method has been successfully applied to determining the content of ATP in injection samples with a RSD of 2.29%.

Key words: Adenosine disodium triphosphate, cerium ions, resonance light scattering

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