TPE-TPP对线粒体显影示意图[53] (A)Chemical structure of TPE-TPP; (B)Fluorescent images of CCCP(10 μmol/L) treated HeLa cells stained with MT(50 nmol/L) for 15 min and TPE-TPP(5 μmol/L) for 30 min. Excitation wavelength: 540~580 nm(for MT) and 330~385 nm(for TPE-TPP); (C)Fluorescent images of CCCP(20 μmol/L) treated living HeLa cells stained with TPE-TPP(5 μmol/L) with increasing scan time(shown in the upper left corner of the panel). Excitation wavelength:405 nm; emission filter:449~520 nm; irradiation time: 15.49 s/scan |