A 3D Glucose Electrochemical Sensor Integrated with Collagen Hydrogel for Real-Time Monitoring of Cell Metabolism

doi:10.19894/j.issn.1000-0518.230260

Chinese Journal of Applied Chemistry ›› 2024, Vol. 41 ›› Issue (1): 156-163.DOI: 10.19894/j.issn.1000-0518.230260

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A 3D Glucose Electrochemical Sensor Integrated with Collagen Hydrogel for Real-Time Monitoring of Cell Metabolism

Chun-Ying YAO¹, Xiao-Yan ZHU², Yan-Ling LIU¹()

^1.College of Chemistry and Molecular Sciences，Wuhan University，Wuhan 430072，China
^2.School of Food Science and Technology，Wuhan Business University，Wuhan 430056，China

Received:2023-08-30 Accepted:2023-11-18 Published:2024-01-01 Online:2024-01-30
Contact: Yan-Ling LIU
About author:yanlingliu@whu.edu.cn
Supported by:
the National Natural Science Foundation of China(22122408)

Abstract

Abstract:

Real-time monitoring of glucose metabolism can not only provide information on cell growth status， but also contribute to the deep understanding of the mechanism of cell metabolism. Electrochemical sensors have become an important tool in real-time detection of glucose. However， most reported glucose sensors are based on two-dimensional electrodes that are hardly compatible with three-dimensional cell culture systems for accurate detection. Herein， a 3D glucose electrochemical sensor with high sensitivity and selectivity is prepared via immobilizing glucose oxidase （GOD） on the surface of a porous electrode which could catalyze H₂O₂ reduction. Then， collagen hydrogel with tumor cells （MCF-7） therein is filled into the pores of the 3D sensor， and thus an integrated platform of 3D cell culture for electrochemical detection is constructed. This platform allows real-time monitoring of the change in glucose concentration in culture medium when MCF-7 cells are cultured under different conditions， providing a new method for evaluating the cell metabolism activities.

Key words: 3D sensor, Electrochemical detection, Cell culture, Glucose metabolism

CLC Number:

O657.1

Chun-Ying YAO, Xiao-Yan ZHU, Yan-Ling LIU. A 3D Glucose Electrochemical Sensor Integrated with Collagen Hydrogel for Real-Time Monitoring of Cell Metabolism[J]. Chinese Journal of Applied Chemistry, 2024, 41(1): 156-163.

Figures/Tables 8

Fig.1 Schematic illustration of the fabrication of 3D glucose electrochemical sensor and the detection principle of glucose metabolism in 3D cell culture

Fig.2 SEM images at different magnifications for （A， B） PCP electrode and （C， D） GOD/PCP electrode

Fig.3 FT-IR spectra of PCP electrode and GOD/PCP electrode

Fig.4 CVs of （A） PCP electrode and （B） GOD/PCP electrode in PBS （black） and 2 mmol/L glucose （red） solution with a scan rate of 10 mV/s

Fig.5 （A） Amperometric responses of GOD/PCP electrode to a series of increasing glucose concentrations in PBS solution at a potential of 0.0 V（vs.Ag/AgCl）；（B） Corresponding calibration curve of GOD/PCP electrode to glucose；（C） Statistical results of amperometric response of GOD/PCP electrodes to 1.0 mmol/L glucose and 10.0 mmol/L potential interfering substances （n=5）

Fig.6 （A） 3D reconstruction from confocal microscope images of MCF-7 cultured in the integrated platform and labeled with Calcein-AM （green） and PI （red） for cell and Cy3 （yellow） for GOD/PCP electrode；（B） Fluorescent image of MCF-7 cultured in the platform for 24 h and labeled with Calcein-AM and PI

Fig.7 Amperometric response of GOD/PCP platform （A） without （w/o） and （B） with （w/） MCF-7 cells cultured therein；（C） Statistic analysis of the ΔI/I0 during the periods of 0~400 s and 401~3600 s. ***P＜0.001

Fig.8 Amperometric response of GOD/PCP platform before and after MCF-7 were treated with （A） 10 μmol/L paclitaxel and （B） 100 μmol/L paclitaxel；（C） Statistic analysis of the ΔI/I0 during the periods of 0~400 s and 401~3600 s. ns： no significance， ***P＜0.001

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A 3D Glucose Electrochemical Sensor Integrated with Collagen Hydrogel for Real-Time Monitoring of Cell Metabolism

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