Electrochemical Determination of the Specific Genetic Sequence in Transgenic Maize Using Reduced Graphene Oxide and Nano Zirconia Composites as a Platform for Immobilizing DNA

doi:10.11944/j.issn.1000-0518.2019.07.180378

Chinese Journal of Applied Chemistry ›› 2019, Vol. 36 ›› Issue (7): 839-846.DOI: 10.11944/j.issn.1000-0518.2019.07.180378

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Electrochemical Determination of the Specific Genetic Sequence in Transgenic Maize Using Reduced Graphene Oxide and Nano Zirconia Composites as a Platform for Immobilizing DNA

Xueliang WANG^a^*(),Zhaoxia WANG^a,Tao WANG^a,Xiaohui DAI^b

^aCollege of Chemistry and Chemical Engineering,Heze University,Heze, Shandong 274015,China
^bCollege of Chemistry and Material Science,Shandong Agricultural University,Taian,Shandong 271018,China

Received:2018-11-28 Accepted:2019-04-10 Published:2019-07-01 Online:2019-07-02
Contact: Xueliang WANG
Supported by:
Supported by the National Natural Science Foundation of China(No.21105023), the Natural Science Foundation Committee of Shandong Province, China(No.ZR2017MB062, No.ZR2015BL014)

Abstract

Abstract:

The phosphinothricin acetyltransferase(PAT) gene is a kind of exogenous DNA segments indicating genetically modified plants. In this research, a sensitive method for determination of the PAT gene in transgenic maize has been developed using reduced graphene oxide and nano zirconia(nanoZrO₂) composites as a platform for immobilizing DNA probes. First, the graphene oxide was direct electrochemically reduced on a glassy carbon electrode(GCE); then, a layer of nanoZrO₂ was covered on the modified electrode. Last, the DNA probes were immobilized via the affinity between phosphate groups in the DNA probes and the oxygen in nanoZrO₂. The hybridization of the DNA probes with the PAT gene segments was monitored by differential pulse voltammetry(DPV). The sensor was stable, reproducible and sensitive to effectively discriminate the PAT gene in transgenic maize. The detection limit was 2.0×10^-15 mol/L.

Key words: differential pulse voltammetry, reduced graphene oxide, nano zirconia, phosphinothricin acetyltransferase gene, transgenic maize

Xueliang WANG, Zhaoxia WANG, Tao WANG, Xiaohui DAI. Electrochemical Determination of the Specific Genetic Sequence in Transgenic Maize Using Reduced Graphene Oxide and Nano Zirconia Composites as a Platform for Immobilizing DNA[J]. Chinese Journal of Applied Chemistry, 2019, 36(7): 839-846.

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Electrochemical Determination of the Specific Genetic Sequence in Transgenic Maize Using Reduced Graphene Oxide and Nano Zirconia Composites as a Platform for Immobilizing DNA

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