Chinese Journal of Applied Chemistry ›› 2015, Vol. 32 ›› Issue (3): 356-361.DOI: 10.11944/j.issn.1000-0518.2015.03.140196

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A Simple Simultaneous Distillation/Solvent Extraction Method Coupled with Gas Chromatography-Mass Spectrometry for the Analysis of Volatile Oils of Illicium Verum

SUN Yuqiong, XIA Donghai, DONG Xinrong, LI Xia*   

  1. College of Science,Hu'nan Agricultural University,Changsha 410128,China
  • Received:2014-06-04 Accepted:2014-10-29 Online:2015-03-02 Published:2015-03-02
  • Contact: Xia LI
  • Supported by:
    Supported by Hunan Science and Technology Bureau Foundation(No.2011FJ6054)

Abstract:

A simple simultaneous distillation and solvent extraction (SSDE) method was developed and had been applied for sample preparation of the gas chromatography-mass spectrometry (GC-MS) analysis of volatile components from Illicium Verum. The simple apparatus was composed of a round flask, a constant pressure dropping funnel and a condenser. The constituents of the volatile oil obtained by SSDE were compared with those obtained by hydrodistillation method (HD) followed by GC-MS analysis. In terms of GC-MS analysis of volatile oil, the relative standard deviation (RSD) values(n=3) of measured relative contents(>0.1%) of 19 identified compounds are no more than 5.59%. The total content of above components is 98.84% of the volatile oil, in which there are 17 components in 89.5% content with RSD less than 5%. And the yield of volatile oil obtained by SSDE and HD is 7.30% (RSD 2.37%(n=5)) and 6.67% (RSD 5.26%(n=5)), respectively. These results indicate that SSDE is a simple, suitable method for the extraction of volatile oil from Illicium Verum.

 

Key words: simultaneous distillation extraction, hydrodistillation, gas chromatography-mass spectrometry, volatile oil, Illicium Verum

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