应用化学

• 研究论文 • 上一篇    下一篇

对硝基苯酚分子印迹聚合物最佳功能单体的筛选及其水样固相萃取中的应用

克帕亚木·买买提,吐尔洪·买买提*,尤努斯江·吐拉洪,布热比亚·亚合普   

  1. (新疆大学化学化工学院 石油天然气教育部重点实验室 乌鲁木齐 830046)
  • 收稿日期:2013-07-02 修回日期:2013-09-17 出版日期:2014-04-10 发布日期:2014-04-10
  • 通讯作者: 吐尔洪·买买提,副教授; Tel:0991-8582654; Fax:0991-8582809; E-mail:turghunm@gmail.com; 研究方向:分子印迹、光纤传感、荧光免疫分析
  • 基金资助:
    新疆维吾尔自治区自然科学基金(2011211A015)

Screening the Best Functional Monomer for Preparation of Molecularly Imprinted Polymer of 4-Nitrophenol and Its Application on Solid Phase Extraction of Water Sample

MUHAMMAD Kipayem, MUHAMMAD Turghun*, TURAHUN Yunusjan, YAKUP Burabiye   

  1. (College of Chemistry & Chemical Engineering,Xinjiang University,Key Laboratory of
    Oil and Gas Fine Chemicals,Educational Ministry of China,Urumqi 830046,China)
  • Received:2013-07-02 Revised:2013-09-17 Published:2014-04-10 Online:2014-04-10

摘要: 以对硝基苯酚(4-NP)为对象,采用非印迹聚合物(NIP)库筛选法选出乙烯基咪唑为最佳功能单体,通过实验选出乙腈为最合适的聚合溶剂、三甲氧基丙基三甲基丙烯酸酯(TRIM)为交联剂制备分子印迹聚合物。 对固相萃取进行了优化,用pH=2.5的磷酸盐缓冲溶液上样、体积比为45∶55的乙腈和磷酸盐缓冲溶液淋洗、甲醇洗脱条件下,聚合物具有最佳印迹效果。 4-NP分子印迹聚合物的表观吸附量达到5.8 mg/g。 该印迹聚合物对4-NP及其结构类似物苯酚和对氯苯酚的萃取回收率分别为96.0%、78.8%和77.8%,表明具有较高的选择性,还成功地用于自来水样品中4-NP的固相萃取,回收率达到93.1%。 由此可得该方法快速、有效,可用于分子印迹聚合物优化制备。

关键词: 分子印迹聚合物, 固相萃取, 对硝基苯酚, 单体筛选, 替代模板

Abstract: A new method for rational design of molecularly imprinted polymer(MIP) of 4-nitrophenol(4-NP) was established. Using the method, nonimprinted polymer(NIP) library screening, vinylimidazole was selected as the best functional monomer. Acetonitrile and trimethylolpropane trimethacrylate(TRIM) were the best polymerization solvent and crosslinker, respectively. Through the optimization of the solid phase extraction(SPE) profile, the best imprinting effect was achieved in pH=2.5 phosphate buffer, washing with the buffer containing 45% acetonitrile and eluting with methanol. The maximum adsorption capacity of the polymer was 5.8 mg/g. Extraction recoveries of imprinted polymers for 4-NP and its analogs, phenol and 4-chlorophenol, were found to be 96.0%, 78.8% and 77.8%, respectively, proving its high selectivity. It was also successfully used for SPE of 4-NP from spiked tap water with a recovery of 93.1%. The method is proved to be effective and fast for the preparation of MIPs.

Key words: molecularly imprinted polymer, solid phase extraction, nitrophenol, monomer screening, dummy-template

中图分类号: