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纳米TiO2光催化氧化对胃癌细胞周期的影响

文雯1,高晓亚1,张爱平1*,宋志英1,孙彦平2   

  1. (1.山西医科大学药学院 太原 030001；2.太太原理工大学洁净化工研究所 太原 030024)
  • 收稿日期:2011-07-22 修回日期:2011-09-23 出版日期:2012-06-10 发布日期:2012-06-10
  • 通讯作者: 张爱平，教授; Tel:0351-4690071; Fax:0351-4690114; E-mail:zhangap1@163.com; 研究方向：纳米二氧化钛的生物效应
  • 基金资助:
    山西省自然科学基金（2010011048-1）山西医科大学科技创新基金(01200806)资助项目

Effects of Nano-TiO2 Photocatalytic Oxidization on the Cell Cycle of BGC-823 Gastric Cancer Cells

WEN Wen1, GAO Xiaoya1, ZHANG Aiping1*, SONG Zhiying1, SUN Yanping2   

  1. (1.School of Pharmacy,Shanxi Medical University,Taiyuan 030001,China;
    2.Institute of Clean Technique for Chemical Engineering of Taiyuan University of Technology,Taiyuan 030024,China)
  • Received:2011-07-22 Revised:2011-09-23 Published:2012-06-10 Online:2012-06-10

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摘要： 以胃癌细胞系BGC-823为研究对象，采用倒置相差显微镜观察纳米TiO2光催化氧化前后BGC-823细胞形态的变化，通过流式细胞仪考察其对BGC-823细胞凋亡率及细胞周期的影响。 结果表明，纳米TiO2光催化氧化使BGC-823细胞呈受激坏死或凋亡晚期继发性坏死状态；导致BGC-823细胞周期阻滞于G2/M期并诱导细胞凋亡，从而抑制其DNA合成及增殖分化进程。 当TiO2比负载量为9.8×10-7 g/cm2、光照时间为40 min时，G2/M期阻滞较为显著，细胞凋亡率达24.34%。

关键词: 光催化氧化, 纳米TiO2, 胃癌细胞BGC-823, 细胞周期, 细胞凋亡

Abstract: In order to explore the mechanisms of killing cancer cells via nano-TiO2 photocatalytic oxidization, gastric cancer cell line BGC-823 was cultured as research object in this study. The morphologic changes were observed by inverted phase-contrast microscope, the apoptotic rates and cell cycle of BGC-823 cells were examined by flow cytometer. The results showed that nano-TiO2 photocatalytic oxidation caused the necrosis or secondary necrosis of BGC-823 cells, and inhibited the synthesis of DNA and their proliferation process, through arresting the G2/M phase and inducing apoptosis of BGC-823 cells. Significant arrest was observed and the apoptotic rates of BGC-823 cells reached 24.34% when the coated amount of TiO2 was 9.8×10-7 g/cm2 and the irradiation time was 40 min.

Key words: photocatalytic oxidization, nano-TiO2, gastric cancer cell line BGC-823, cell cycle, apoptosis

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