中华医学超声杂志

应用化学 ›› 2019, Vol. 36 ›› Issue (12): 1456-1461.DOI: 10.11944/j.issn.1000-0518.2019.12.190099

• 研究论文 • 上一篇    下一篇

一种钌(Ⅱ)配合物荧光探针用于半胱氨酸和高半胱氨酸的检测

刘学文ab*(),唐裕才ab   

  1. a湖南文理学院化学与材料工程学院 湖南 常德 415000
    b南京大学配位化学国家重点实验室 南京 210023
  • 收稿日期:2019-04-11 接受日期:2019-07-11 出版日期:2019-12-01 发布日期:2019-12-10
  • 通讯作者: 刘学文
  • 基金资助:
    湖南省教育厅重点项目(16A145)和南京大学配位化学国家重点实验开放课题(SKLCC1920)项目资助

A Luminescent Probe Based on Ruthenium(Ⅱ) Complex for the Detection of Cysteine and Homocysteine

LIU Xuewenab*(),TANG Yucaiab   

  1. aCollege of Chemistry and Material Engineering,Hunan University of Arts and Science,Changde,Hunan 415000,China
    bState Key Laboratory of Coordination Chemistry,School of Chemistry and Chemical Engineering,Nanjing University,Nanjing 210023,China
  • Received:2019-04-11 Accepted:2019-07-11 Published:2019-12-01 Online:2019-12-10
  • Contact: LIU Xuewen
  • Supported by:
    Supported by the Research Foundation of Education Bureau of Hunan Province(No.16A145), and the State Key Laboratory of Coordination Chemistry(No.SKLCC1920)

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摘要：

为了检测半胱氨酸和高半胱氨酸,本文合成了一种基于钌(Ⅱ)配合物的荧光探针。 结果表明,该探针可实现对半胱氨酸和高半胱氨酸的较好的灵敏性和选择性检测。 在优化的实验条件下,5~35 μmol/L浓度区间,探针的荧光强度与半胱氨酸和高半胱氨酸浓度呈良好的线性关系。 其检测限分别为0.60和0.78 μmol/L。 该研究为基于钌(Ⅱ)配合物的荧光探针定量检测生物活性分子提供了一种有用的方法。

关键词: 半胱氨酸, 高半胱氨酸, 钌(Ⅱ)配合物;, 荧光探针

Abstract:

A new turn-on type of luminescence probe based on ruthenium(Ⅱ) complex, [Ru(5-CHO-phen)3]2+(5-CHO-phen=1,10-phenanthroline-5-carbaldehyde), has been designed and synthesized. The probe displayed good sensitivity and selectivity toward cysteine (Cys) and homocysteine (Hcy). Enhanced luminescence intensity of this probe displays a good linear relationship in the concentration range of 5 to 35 μmol/L for Cys and Hcy. The detection limit is 0.60 μmol/L and 0.78 μmol/L for Cys and Hcy, respectively. The proposed approach provides a useful method by using luminescence probe based on ruthenoium(Ⅱ) complex for the quantitative detection of various biological molecules.

Key words: cysteine, homocysteine, ruthenium(Ⅱ) complex;, luminescent probe

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