Abstract: A new carbamate functionalized ionic liquid stationary phase(ImPh-Carb-Silica) for high-performance liquid chromatography was prepared by bonding 4,4′-diphenylmethylene-bis[2-(1-imidazolyl)-3-phenyl-1-propanol carbamate](ImPh-Carb) onto silica particles. The structure of ImPh-Carb was characterized by 1H NMR,13C NMR, MS and FTIR, and the bonded stationary phase was also characterized by FTIR and element analysis, and the bonding amount of ImPh-Carb-Silica stationary phase was about 0.19 mmol/g, according to the nitrogen content. The chromatographic performances of the stationary phase were evaluated using aromatic hydrocarbons, phenols and organophosphorus pesticides as solutes in reversed-phase and normal-phase chromatographic modes, and the effect of the content of mobile phase on retention factor(lg k) of analytes was studied. The results show that the functionalized ionic liquid stationary phase provides a multiple-interaction mechanism with analytes including hydrophobic, hydrogen bonding, π-π, and dipole-induce/dipole interactions. The stationary phase can be simultaneously performed in reversed-phase and normal-phase chromatographic modes, and it demonstrates its superiority in separation of phenols and organophosphorus pesticides under normal-phase chromatographic mode.

Key words: carbamate functionalized ionic liquid, stationary phase, high-performance liquid chromatography, multiple-interaction mechanism